Discover the affects of PH on the enzyme catalase

Catalase- This is an enzyme implant in food such as white tater vine and liver. It is officed for removing henry hydrogen peroxide from cells. Hydrogen peroxide is the poisonous byproduct of metabolism. It speeds up the decomposition of hydrogen Peroxide into oxygen and water. It is able to do this be ground the require of its active voice site matches the shape of Hydrogen Peroxide pinpoint. This type of chemical reaction where a molecule is broken overmatch into little pieces is an ANABOLIC REACTION.BACKGROUND INFORMATION ON ENZYMESEnzymes- Enzymes ar complex 3-D global proteins, some of which devour different associated molecules. Enzymes are a catalyst which alters the rate of chemical reaction with step up itself undergoing a permanent change, and thus can be utilise oer and over again.Enzymes help reactions speed up which would otherwise take place genuinely slowly.While the enzyme molecule is relatively large than the larger than the substrate molecule it acts upon, solitary(prenominal) a sm and part of the enzyme molecule actu entirelyy comes into contact. This region is called the ACTIVE SITE. The active site of an enzyme is the region that binds the substrate and contributes the amino group acid residues that directly participate in the making and breaking of chemical bonds. However, all enzymes operate alone on a specific shape and in that respectfore fail only complementary locks, so only substrates of a particular shape ravelament fit the active site of an enzyme. expectancyI predict that as the PH increases the activity of enzyme increases until the best PH is reached. Catalase best ph is around 6-7.after that the enzyme allow be denatured.HypothesisThe enzyme will take a shit an optimum pH of around neutral. Either nerve of the optimum pH will lease relatively high rates of reaction or far away from the optimum pH will have a low rate of reaction. The enzyme catalase is found in m whatever tissues including stu mp spud and liver. It is important because it breaks down the hydrogen peroxide formed in metabolism. Hydrogen peroxide is toxic and if it were non broken down it would kill the cells. As it does this, it liberates carbon dioxide that can be collected. chemical EQUATION2H2O2 - 2H2O + O2I also muse that the catalase activity will be highest with the judge of potato comparing to the apple.VariablesThe factors, which will have an carry on on the enzyme activity, will be* Temperature* Concentration of enzymes* pHThe variable in which I will be exploitation to investigate the enzyme activity is the temperature. The rest of the variables will be kept constant and only the temperatures will be changed.ApparatusTo test how the PH affects the rate of reaction, I will use the following apparatus* muck up syringe through bung* tater* Hydrogen Peroxide* Stop clock* Cork borer* Scalpel* water Beaker-250 cm* Delivery Tube* Ph 3,5,7,9* 5 cm or 10 cm plastic syringe with barricade whoop o ff* quantity cylinder* conelike flask* White tile* mount* Test bathtubes* Test tube extort* Safety equipment goggles, lab coat, glovesAlternative substance abuse yeast, broad beans or liver, instead of potato and apple.Method1. evaluate out 5cm3 of potato into a conical flask2. quantify out 10 cm3 of buffer resolvent into a separate container and mix it with minced potato.3. Measure out 5cm3 volume of hydrogen peroxide in syringe.4. Fill a tub with water.5. Completely immerse a measuring cylinder in the water making sure to release all publicise bubbles in the cylinder and keep underwater.6. impersonate the cylinder upright ensuring the open end remains underwater and clamp steadfastly7. Add the hydrogen peroxide to the potato and place bung on conical flask whilst starting timer.8. Run essay for 5 minutes taking readings all 30 seconds by noting the amount of gas at top of cylinder (at meniscus)9. accept the examine with different ph.Justification of mannerI have chosen to use this method because it is safe, efficient and accurate. I disembodied spirit that it will help me to obtain rock-steady results.Risk Assessment* Hydrogen peroxide is a dangerous substance. It can cause serious injury to the eyes and skin. It is therefore necessary that goggles be used for eye protection. In addition, eye irrigation equipment should be readily available in effect of an emergency.* The works area must be clean, tidy and dry. Make sure there is no obstacle in the working area so that you can easy move around as. This allows you to perform the experiment safely and unimpeded care should be taken to ensure that liquid is not spilled onto the floor to cause slippage.Result plug-inTime(s)Oxygen gas collected (cm3)Ph 3 ph 5 ph7 ph930 1.5 1.5 0.5 1.560 1.5 3.0 1.5 2.090 1.5 5.0 2.0 2.0120 1.5 7.0 5.65 2.0cl 1.5 8.3 10.3 2.0180 1.5 9.0 14.35 2.0210 1.5 10.0 17.35 2.0240 1.5 11.1 20.15 2.0270 1.5 13.6 24.15 2.0300 1.5 14.0 31.15 2.0These results clarify that the optimum ph for catalase is 7 which supports my prediction. At the worst ph 3, less oxygen was released, measuring a slower rate of reaction. My emphasize information explains this. The enzyme dont work as it is neared extremum PHS. This is because its shape would have been change roughly means the active site may not fit into the substrate any more.Further investigationIf this investigation were to be done again, I would take all results myself and use my own interpretation, thus gaining more reliable results. I will get laid how much potato to measure out for each experiment and also I will make sure that the other variables are kept constant. I have taken the same amount of each, tutelage them constant. This would give me clearer results to aid me in my collar as to how ph can affect the activity of catalase.